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教授



谢希贤理学博士,教授,博士生导师。入选国家“万人计划”领军人才,科技部中青年科技创新领军人才,现任代谢控制发酵技术国家地方联合工程实验室副主任,天津微生物学会理事,天津市食品学会理事。

 

教育科研背景:

[1] 2012.32013.3     Rice University, USA                访问学者

[2] 2004.92007.7 厦门大学生命科学学院       理学博士

[3] 2001.92004.7 国家海洋局第三海洋研究所          理学硕士

[4] 1995.92000.7 中国科学技术大学生命科学学院    理学学士

 

主讲课程

[1] 本科生课程:代谢控制发酵

[2] 研究生课程:代谢工程

 

科研领域及方向

[1] 代谢工程:氨基酸和核苷酸及衍生物代谢工程育种、代谢网络定量分析、氨基酸和核苷酸及衍生物产品开发和应用;

[2] 发酵工程:发酵过程优化放大,产品分离提取,节能减排和资源高效利用;

[3] 系统生物学:重要工业微生物生理代谢、基因组和蛋白质组等比较组学研究。

 

科研情况

[1] 发酵法生产核苷类药物利巴韦林,天津市科技支撑计划项目,2016.4-2019.3,项目负责人。

[2] 谷氨酸棒杆菌分支链氨基酸代谢网络比较分析及动态调控研究,国家自然科学基金项目,2015.01-2018.12,项目负责人。

[3] 有机酸生物合成途径构建与优化技术,国家863计划项目,2015.1-2017.12,子课题负责人。

[4] 低温嘌呤核苷磷酸化酶在利巴韦林发酵中的应用基础研究,国家自然科学基金项目,2012.1-2014.12,项目负责人。

[5] 抗生素、维生素、氨基酸等大宗产品的微生物发酵及分离纯化技术,国家科技支撑计划项目,2008.7-2010.12,子课题负责人。

[6] 5万吨/年谷氨酸元素循环酸碱再生耦联技术与示范,国家科技支撑计划项目,2011.1-2013.12,子课题负责人。

[7] 甜菜碱等产酸促进剂对氨基酸发酵影响的研究,发酵行业共性技术开发专项,2010.11-2011,课题负责人。

[8] 利用黄色短杆菌高效生产L-缬氨酸,天津市科技支撑计划项目,2008.4-2011.3,项目负责人。

[9] 微生物发酵法生产利巴韦林的研究,天津市教委基金项目,2007.11-2010.10,项目负责人。

[10] 生物发酵法生产组氨酸技术,横向课题,2017.12-2027.12,项目负责人。

[11] 生物发酵法生产丝氨酸技术,横向课题,2018.8-2038.7,项目负责人。

[12] 高产L-色氨酸菌株委托开发,横向课题,2018.5-2019.12,项目负责人。

[13] 高产精氨酸和瓜氨酸发酵技术开发,横向课题,2018.7-2020.7,项目负责人。

[14] 氨基酸生产菌株高产及抗逆机制发现与菌株选育,横向课题,2014.10-2015.10,项目负责人。

[15] 苏氨酸或色氨酸前体或衍生物开发,横向课题,2015.5-2016.5,项目负责人。

[16] L-缬氨酸发酵生产技术的开发,横向课题,2010.7-2011.7,项目负责人。

[17] 鸟苷发酵生产技术的开发,横向课题,2008.11-2010.11,项目负责人。

 

代表性论文/著作

[1] Ning Y, Wu X, Zhang C, Xu Q, Chen N, Xie X*. Pathway construction and metabolic engineering for fermentative production of ectoine in Escherichia coli. Metab Eng, 2016, 36:10-18.  IF: 8.201

[2] Wu H, Li Y, Ma Q, Li Q, Jia Z, Yang B, Xu Q, Fan X, Zhang C, Chen N, Xie X*. Metabolic engineering of Escherichia coli for high-yield uridine production. Metab Eng, 2018, 49:248-256.  IF: 8.201

[3] Zhang C, Li Y, Ma J, Liu Y, He J, Li Y, Zhu F, Meng J, Zhan J, Li Z, Zhao L, Ma Q, Fan X, Xu Q, Xie X, Chen N*. High production of 4-hydroxyisoleucine in Corynebacterium glutamicum by multistep metabolic engineering. Metab Eng, 2018, 49:287-298.  IF: 8.201

[4] Fan X, Wu H, Jia Z, Li G, Li Q, Chen N, Xie X*. Metabolic engineering of Bacillus subtilis for the co-production of uridine and acetoin. Appl Microbiol Biotechnol, 2018, 102(20):8753-8762.  IF: 3.340

[5] Zhang C, Qi J, Li Y, Fan X, Xu Q, Chen N, Xie X*. Production of α-ketobutyrate using engineered Escherichia coli via temperature shift. Biotechnol Bioeng, 2016, 113(9):2054-9.  IF: 4.126

[6] Li Y, Wei H, Wang T, Xu Q, Zhang C, Fan X, Ma Q, Chen N, Xie X*. Current status on metabolic engineering for the production of l-aspartate family amino acids and derivatives. Bioresour Technol, 2017, 245:1588-1602.  IF: 5.651

[7] Ma Y, Chen Q, Cui Y, Du L, Shi T, Xu Q, Ma Q, Xie X, Chen N. Comparative genomic and genetic functional analysis of industrial L-leucine- and L-valine-producing Corynebacterium glutamicum strains. J Microbiol Biotechnol, 2018, doi: 10.4014/jmn.1805.05013.  IF: 1.650

[8] Fan X, Wu H, Li G, Yuan H, Zhang H, Li Y, Xie X*, Chen N. Improvement of uridine production of Bacillus subtilis by atmospheric and room temperature plasma mutagenesis and high-throughput screening. PLoS One, 2017, 12(5):e0176545.  IF: 3.234

[9] Qian Ma, Quanwei Zhang, Qingyang Xu, Chenglin Zhang, Yanjun Li, Xiaoguang Fan, Xixian Xie*, Ning Chen, Systems metabolic engineering strategies for the production of amino acids. Synth Syst Biotechnol, 2017, 2: 87-96.

[10] Gui Y, Ma Y, Xu Q, Zhang C, Xie X*, Chen N. Complete genome sequence of Corynebacterium glutamicum CP, a Chinese l-leucine producing strain. J Biotechnol, 2016, 220:64-65.  IF: 2.871

[11] Li Y, Sun L, Feng J, Wu R, Xu Q, Zhang C, Chen N, Xie X*. Efficient production of α-ketoglutarate in the gdh deleted Corynebacterium glutamicum by novel double-phase pH and biotin control strategy. Bioprocess Biosys Eng, 2016, 39(6):967-76.  IF: 1.997

[12] Xie X, Liang Y, Liu H, Liu Y, Xu Q, Zhang C, Chen N*. Modification of glycolysis and its effect on the production of L-threonine in Escherichia coli. J Ind Microbiol Biotechnol, 2014, 41:1007-1015.  IF: 2.439

[13] Wang J, Wen B, Wang Jian, Xu Q, Zhang C, Chen N, Xie X*. Enhancing l-isoleucine production by thrABC overexpression combined with alaT deletion in Corynebacterium glutamicum. Appl Biochem Biotech, 2013, 171(1):20-30.   IF: 1.893

[14] Guo X, Wang J, Xie X, Xu Q, Zhang C, Chen N*. Enhancing the supply of oxaloacetate for L-glutamate production by pyc overexpression in different Corynebacterium glutamicum. Biotechnol Lett. 2013, 35(6):943-950. IF: 1.736

[15] Liang J, Zhang D, Guo X, Xu Q, Xie X, Zhang C, Bai G, Xiao X, Chen N*. At-line near-infrared spectroscopy for monitoring concentrations in temperature-triggered glutamate fermentation. Bioprocess Biosyst Eng. 2013, 36(12):1879-1887.  IF: 1.823

[16] Fang H, Xie X, Xu Q, Zhang C, Chen N*. Enhancement of cytidine production by coexpression of gnd, zwf, and prs genes in recombinant Escherichia coli CYT15. Biotechnol Lett. 2013, 35(2):245-251. IF: 1.736

[17] Liu Q, Cheng Y, Xie X, Xu Q, Chen N*. Modification of tryptophan transport system and its impact on production of L-tryptophan in Escherichia coli. Bioresour Technol. 2012, 114:549-554.  IF: 4.75

[18] Xie X, Huo W, Xia J, Xu Q, Chen N*. Structure-activity relationship of a cold-adapted purine nucleoside phosphorylase by site-directed mutagenesis. Enzyme Microb Technol. 2012, 51(1):59-65.  IF: 2.592

[19] Xie X, Xu L, Shi J, Xu Q, Chen N*. Effect of transport proteins on L-isoleucine production with the L-isoleucine-producing strain Corynebacterium glutamicum YILW. J Ind Microbiol Biotechnol. 2012, 39(10): 1549-1556.  IF: 2.321

[20] Shen T, Liu Q, Xie X, Xu Q, Chen N*. Improved production of tryptophan in genetically engineered Escherichia coli with TktA and PpsA overexpression. J Biomed Biotechnol. 2012, 2012:605219.  IF: 2.88

[21] Xie X, Xia J, Xu Q, He K, Lu L, Chen N*. Low-molecular-mass homotrimer purine nucleotide phosphorylase: characterization and application in enzymatic synthesis of nucleoside antiviral drugs. Biotechnol Lett. 2011, 33(6):1107-1112. IF: 1.683

[22] Xie X, Wang G, Xia J, Chen N. Characterization of a recombinant cold-adapted purine nucleoside phosphorylase and its application in ribavirin bioconversion. World J Microbiol Biotechnol. 2011, 27:1175-1181.  IF: 1.532

 

发明和申请专利

[1] Hydroxy-and dicarboxylic-fat synthesis by microbes, US 2015/0225753 A1.

[2] Xylose-induced genetically engineered bacteria used for producing ectoine and use thereof, PCT/CN2017/088284.

[3] Genetically engineered bacteria with high-yield uridine and its construction method and use, PCT/CN2018/072020.

[4] 高产尿苷的基因工程菌及其构建方法与应用,ZL2018100209443

[5] 过表达异源谷氨酰胺合成酶的基因工程菌及构建方法,ZL201711031742.0

[6] 利用木糖诱导产生四氢嘧啶的基因工程菌及其应用,ZL2017100128456

[7] 一种联产尿苷和乙偶姻的发酵生产方法,ZL201610137185.X

[8] 高产嘧啶核苷的菌株及其氨甲酰磷酸合成酶调节位点,ZL201511035070.1

[9] 一株高产嘧啶核苷的菌株及其氨甲酰磷酸合成酶调节位点,ZL201511035141.8

[10] 一种产生四氢嘧啶的基因工程菌及其构建方法与应用ZL201510410080.2

[11] 一种色氨酸连续发酵分离装置,ZL201620734548.3

[12] 一种膜法提取发酵液中L-色氨酸的新工艺,ZL201510653963.6

[13] 一种双膜连续结晶法分离提取发酵液中分支链氨基酸的方法,ZL201510880406.8

[14] 一株产α-酮基丁酸的基因工程菌及其应用ZL201410132996.1

[15] 利用重组表达核糖磷酸转移酶生产5-肌苷酸的方法,ZL201410032597.8

[16] 一种L-异亮氨酸羟化酶基因及其基因工程菌与应用,ZL201410132444.0

[17] 解淀粉芽孢杆菌添加前体物发酵法生产抗病毒药物利巴韦林的生产工艺, ZL201310097836.3

[18] 谷氨酸棒杆菌及采用该菌发酵生产α-酮戊二酸的方法,ZL201110392778.8

 

获奖情况

[1] 玉米原料高效清洁生产谷氨酸关键技术与产业化,黑龙江省,科技进步奖,一等奖,2017

[2] 甜菜碱提高氨基酸生产关键技术及产业化,中国轻工业联合会,科技进步奖,一等奖,2017

[3] 谷氨酸温度敏感突变株代谢调控关键技术及产业化,中国商业联合会,科技进步奖,特等奖,2017

[4] 伦世仪教育基金杰出青年学者,伦世仪教育基金理事会,2016

[5] 芳香族氨基酸及衍生物关键技术研究与产业化,中国轻工业联合会,科技进步奖,一等奖,2015

[6] 谷氨酸清洁生产和废水综合利用关键技术研究与应用,中国轻工业联合会,科技进步奖,二等奖,2015

[7] 分支链氨基酸代谢调控技术及产业化,天津市,科技进步奖,二等奖,2013

 

联系方式

办公地点:天津经济技术开发区第十三大街29号,亚博APP手机版,邮编:300457

办公电话:022-60601251

Email: xixianxie@tust.edu.cn