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教授




黎明,男,博士,教授,硕士生导师。2003年09至今一直亚博APP手机版、教育部工业发酵微生物重点实验室、天津市工业微生物重点实验从事教学和科研工作。曾在美国伦斯勒理工学院访学一年。

科研方向:
[1] 微生物与生化药学:基因工程药物,酶制剂的开发与应用;
[2] 工业微生物育种:基于代谢工程策略,对微生物进行改造,从而实现高附加值化学品和平台化合物的微生物合成与生产;
[3] 发酵工程:微生物代谢工程与发酵。


科研项目:
参与或主持的项目多项,近五年的主要科研项目如下:
1、国家自然科学基金面上项目,No.21176190、谷氨酸棒杆菌生物合成尸胺及其代谢调控研究。主持人
2、天津市科技支撑计划项目,No. 11ZCKFSY00900、高活力碱性果胶酶的规模发酵技术及酶制剂的开发。主持人
3、天津市应用基础及前沿技术研究计划项目,No. 11JCYBJC09600、高产α-葡萄糖转苷酶黑曲霉工程构建及发酵优化。主持人。
4、国家863项目,NO.2007AA02Z212、热稳定低温碱性蛋白酶的定向改造。第3完成人。
5、国家科技部项目子项目:食品微生物基因组改造,第三参加人。
6、国家科技部项目子项目:大宗食品发酵安全低碳加工关键技术研究-07.第二参加人。
7、教育部项目:大肠杆菌中蒜氨酸生物合成途径的构建及其代谢流研究。第二参加人。
8、亚博网页版登录科研平台项目,高产柠檬酸大肠杆菌工程菌的构建。主持人。

代表性论文:
近五年,发表论文22篇,SCI收录9篇,EI收录6篇,申请专利6项,授权专利2项。主要论文如下:
1.Wenqin He, Yuanyuan Zhu, Abhijeet Shirke, Xiaojun Sun, Jian Liu, Richard A. Gross, Mattheos A. G. Koffas*, Robert J. Linhardt*, Ming Li*. Expression of chondroitin-4-O-sulfotransferase in Escherichia coli and Pichia pastoris. Applied Microbiology and Biotechnology(Appl Microbiol Biotechnol). 2017, 101: 6919-6928.
2.杨田, 随树珍, 刘逸寒, 黎明*.大麻纤维微生物脱胶菌株的筛选、鉴定及其应用. 亚博网页版登录学报, 2017,32(4):24-29.
3.Qing Hao, Xiaoguang Liu, Guozhong Zhao*, Lu Jiang, Ming Li, Bin Zeng*. Recombinant expression, purification, and characterization of an acyl-CoA binding protein from Aspergillus oryzae. Biotechnology Letters (Biotechnol Lett). 2016, 38:519–525
4.Run Wei, Xiao-mei Liang1, Mei-juan Xuan, Hao-yu Yuan, Fu-ping Lu and Ming Li*. Construction of the Escherichia coli-Bacillus subtilis Shuttle Vector pBE2R and Identification of the Critical Residues Involved in the Autoprocessing of the Propeptide of the Alkaline Protease. International Conference on Applied Biotechnology (ICAB2016), November 25-27, 2016. Tianjin, China.
5.Mei-juan Xuan, Jian-zhong Zhang, Hao-yu Yuan, Run Wei, Fu-ping Lu and Ming Li*. Optimization of Process for Optical Resolution of DL-pantolactone Using Immobilized D-lactonohydrolase. International Conference on Applied Biotechnology (ICAB2016), November 25-27, 2016. Tianjin, China.
6.黎明,唐奇,李东霞,李杨,胡顿吉,随树珍,路福平. 谷氨酸棒杆菌发酵生产1,5-戊二胺的代谢流分析. 亚博网页版登录学报. 2015,30(1):9-13
7.Ming Li, Dongxia Li, Yunyan Huang, Meng Liu, Hongxin Wang, Qi Tang, Fuping Lu. Improving the secretion of cadaverine in Corynebacterium glutamicum by cadaverine-lysine antiporter. Journal of Industrial Microbiology & Biotechnology. 2014, 41(4): 701-709.
8.李东霞, 黎明*, 王洪鑫, 王舒雅, 路福平. 生物法合成戊二胺研究进展. 生物工程学报. 2014, 30(2):161-174.
9.Ming LI, Liying ZHOU, Meng LIU, Yunyan HUANG, Xin SUN, Fuping LU. The Construction of Engineering Strain producing high yield of α-transglucosidase via Agrobacterium tumefaciens-mediated Transformation of Asperillus niger. Bioscience Biotechnology and Biochemis.2013, 77(9):1860-1866
10.Ming Li, Dongxia Li, Yunyan Huang, Tao Niu, Meng Liu, Liying Zhou, Xin Sun, Fuping Lu. Construction of Recombinant Corynebacterium glutamicum Producing Cadaverine by One Step Method. International Journal of Advancements in Computing Technology. 2013, 5(8): 619-629.
11.Tonglei Shi, Aiqun Yu, Ming Li, Meng Zhang, Laijun Xing, Mingchun Li. Identification and characterization of a novel C20-elongase gene from the marine microalgae, Pavlova viridis, and its use for the reconstitution of two pathways of long-chain polyunsatured fatty acids biosynthesis in Saccharomyces cerevisiae. Biotechnology Letters. 2013,35(8):1271-1282.
12.Ming Li, Xiuyuan Ou, Dongsheng Wei, Xiangdong Yang, Dongquan Guo, Xueyan Qian, Laijun Xing, Mingchun Li. Cloning and identification of a novel Δ9-specific polyunsaturated fatty acid elongase variant from DHA-producing Isochrysis galbana H29. Biotechnology and Bioprocess Engineering. 2012,17:22-32.
13.Tonglei Shi, Aiqun Yu, Ming Li, Xiuyuan Ou, Laijun Xing and Mingchun Li. Identification of a novel C22-∆4-producing docosahexaenoic acid (DHA) specific polyunsaturated fatty acid desaturase gene from Isochrysis galbana and its expression in Saccharomyces cerevisiae. Biotechnology Letters. 2012,34:2265-2274 
14.Yihan Liu, Guanqun Chen, Jianling Wang, Yujie Hao, Ming Li, Yu Li, Bo Hu, Fuping Lu. Efficient expression of an alkaline pectate lyase gene from Bacillus subtilis and the characterization of the recombinant protein. Biotechnol Lett,2012,34:109–115
15.黎明,刘萌,黄云雁,周丽颖,孙昕,路福平.根癌农杆菌介导的黑曲霉遗传转化体系的建立及优化.中国生物工程杂志.2012,32(1):56-63.
16.Ming Li, Xiuyuan Ou, Xiangdong Yang, Dongquan Guo, Xueyan Qian, Laijun Xing, and Mingchun Li. Isolation of a novel C18-Δ9 polyunsaturated fatty acid specific elongase gene from DHA-producing Isochrysis galbana H29 and its use for the reconstitution of the alternative Δ8 pathway in Saccharomyces cerevisiae. Biotechnology Letters. 2011, 33(9): 1823-1830

主要著作:
参加编写《微生物实验技术》,中国轻工业出版社,2005.


获奖成果:
“载体药物中间体高浓度生物转化技术”,津市科技进步二等奖。


联系方式:liming09@tust.edu.cn